Directed transport of singlet excitation power is a key course of in pure light-harvesting methods and a desired function in assemblies of purposeful natural molecules for natural electronics and nanotechnology functions. Nevertheless, progress on this course is hampered by the shortage of ideas and mannequin methods. Right here we show an all-optical method to govern singlet exciton transport pathways inside supramolecular nanostructures by way of singlet–triplet annihilation, i.e., to implement an efficient movement of singlet excitons alongside a predefined course. For this proof-of-concept, we domestically photo-generate a long-lived triplet exciton inhabitants and subsequently a singlet exciton inhabitants on single bundles of H-type supramolecular nanofibres utilizing two temporally and spatially separated laser pulses. The native triplet exciton inhabitants operates as a gate for the singlet exciton transport since singlet–triplet annihilation hinders singlet exciton movement throughout the triplet inhabitants. We visualize this manipulation of singlet exciton transport by way of the fluorescence sign from the singlet excitons, utilizing a detection-beam scanning method mixed with time-correlated single-photon counting. Our reversible, all-optical manipulation of singlet exciton transport can pave the best way to realising new design rules for purposeful photonic nanodevices.